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Home >> Antibodies >> Recombinant Human IgG2 Antibody (Biotin Conjugate)

Recombinant Human IgG2 Antibody (Biotin Conjugate) [clone RM118] (R20188BTN)

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  Catalog No Formulation Size Price (USD)  
Image R20188BTN-50UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 50 ug 419
Microvalidated Recrabbitmono
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Sandwich ELISA with human IgG2 using recombinant Human IgG2 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
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Sandwich ELISA with human IgG2 using recombinant Human IgG2 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
Sandwich ELISA with human plasma using recombinant Human IgG2 antibody as the capture, and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
ELISA of human immunoglobulins shows recombinant Human IgG2 antibody only reacted to hIgG2. No cross reactivity with IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse/rat/goat IgG.
ELISA Titration: the plate was coated with different amounts of human IgG2. A serial dilution of recombinant Human IgG2 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Availability 1-3 business days
Species Reactivity Human
Format Biotin Conjugate
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM118
Purity Protein A purified from animal origin-free supernatant
UniProt P01859
Gene ID 3501
Applications ELISA : 50ng/well-200ng/well (Capture); 0.05-0.2ug/ml (Detection)
Limitations This recombinant Human IgG2 antibody is available for research use only.
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Description

This recombinant Human IgG2 antibody reacts to the heavy chain of human IgG2. No cross reactivity with human IgG1, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

Human IgG2 was used as the immunogen for this recombinant Human IgG2 antibody.

Storage

Store the recombinant Human IgG2 antibody at -20oC.

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