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Home >> Antibodies >> Recombinant Histone H3 Antibody

Recombinant Histone H3 Antibody [clone RM188] (R20252)

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  Catalog No Formulation Size Price (USD)  
Image R20252-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
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R20252-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 239
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Western blot of recombinant Histone H3.3 and H3.1 proteins, A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using recombinant Histone H3 antibody at 0.025 ug/ml.
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Western blot of recombinant Histone H3.3 and H3.1 proteins, A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using recombinant Histone H3 antibody at 0.025 ug/ml.
ICC/IF of HeLa cells using recombinant Histone H3 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Sandwich ELISA against acetylated Histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with sodium butyrate, using recombinant Histone H3 antibody (1ug/ml) as the capture and biotinylated anti-H3K9ac (RM161, 1ug/ml) as the detect.
Sandwich ELISA against acetylated Histone H3 at Lys 79 using HeLa whole cell lysate, treated or untreated with sodium butyrate, using anti-H3K79ac (RM156, 5 ug/ml) as the capture antibody and biotinylated recombinant Histone H3 antibody (RM188, 1 ug/ml) as the detect.
This recombinant Histone H3 antibody reacts to both Histone H3.1 and H3.3.
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM188
Purity Protein A purified from animal origin-free supernatant
UniProt P84243
Gene ID 8350
Applications Western blot : 0.01-0.25ug/ml
Immunocytochemistry : 1ug/ml-5ug/ml
ELISA : 0.2-1ug/ml
Limitations This recombinant Histone H3 antibody is available for research use only.
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Description

This recombinant Histone H3 antibody reacts to all Histone H3 proteins, independent of post-translational modifications. No cross reactivity with other Histone proteins.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Histone H3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A peptide corresponding to the C-terminus of human Histone H3 was used as the immunogen for this recombinant Histone H3 antibody.

Storage

Store the recombinant Histone H3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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