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Home >> Antibodies >> Recombinant H4K5ac Antibody

Recombinant H4K5ac Antibody [clone RM199] (R20224)

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  Catalog No Formulation Size Price (USD)  
Image R20224-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Image
R20224-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 239
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Western blot test of (1) acid extracts of HeLa cells and (2) recombinant Histone H4 recombinant H4K5ac antibody at 0.2 ug/ml showed a band of Histone H4 acetylated at Lysine 5 in HeLa cells.
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Western blot test of (1) acid extracts of HeLa cells and (2) recombinant Histone H4 recombinant H4K5ac antibody at 0.2 ug/ml showed a band of Histone H4 acetylated at Lysine 5 in HeLa cells.
The recombinant H4K5ac antibody specifically reacts to Histone H4 acetylated at Lysine 5 (K5ac). No cross reactivity with unmodified Lysine 5 or other acetylated Lysines.
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H4K5ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM199
Purity Protein A purified from animal origin-free supernatant
UniProt P62805
Gene ID 121504
Applications Western blot : 0.2-1ug/ml
ELISA : 0.2-1ug/ml
Immunocytochemistry : 0.5-2ug/ml
Limitations This recombinant H4K5ac antibody is available for research use only.
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Description

This recombinant H4K5ac antibody reacts to Histone H4 acetylated at Lysine 5 (K5ac). No cross reactivity with other acetylated Lysines in Histone H4.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H4K5ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys5) was used as the immunogen for this recombinant H4K5ac antibody.

Storage

Store the recombinant H4K5ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Related Products

   1.   View all Recombinant antibodies

   2.   View all H4K5ac antibodies

   3.   View all Histone H4 antibodies

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