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Home >> Antibodies >> Recombinant H4K20me3 Antibody

Recombinant H4K20me3 Antibody [clone RM208] (R20238)

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  Catalog No Formulation Size Price (USD)  
Image R20238-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Image
R20238-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 239
Microvalidated Recrabbitmono
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Western blot test of (1) acid extracts of HeLa cells and (2) recombinant Histone H4, using recombinant H4K20me3 antibody at 1 ug/ml, showed a band of Histone H4 trimethylated at Lysine 20 in HeLa cells.
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Western blot test of (1) acid extracts of HeLa cells and (2) recombinant Histone H4, using recombinant H4K20me3 antibody at 1 ug/ml, showed a band of Histone H4 trimethylated at Lysine 20 in HeLa cells.
This recombinant H4K20me3 antibody specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). May slightly cross react to dimethylated Lysine 20 (K20me2) at higher concentrations. No cross reactivity with non-modified or monomethylated Lysine 20 (K20me1), or other H4 methylations.
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM208
Purity Protein A purified from animal origin-free supernatant
UniProt P62805
Gene ID 121504
Applications Western blot : 1-2ug/ml
ELISA : 0.5-1ug/ml
Limitations This recombinant H4K20me3 antibody is available for research use only.
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Description

This recombinant H4K20me3 antibody reacts to Histone H4 trimethylated at Lysine 20 (K20me3). It may slightly cross react to dimethylated Lysine 20 (K20me2) at a higher concentration. No cross reactivity with non-modified or monomethylated Lysine 20 (K20me1), or other methylations in histone H4.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H4K20me3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A trimethyl-peptide corresponding to Trimethyl-Histone H4 (Lys20) was used as the immunogen for this recombinant H4K20me3 antibody.

Storage

Store the recombinant H4K20me3 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Related Products

   1.   View all Recombinant antibodies

   2.   View all H4K20me3 antibodies

   3.   View all Histone H4 antibodies

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