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Home >> Antibodies >> Recombinant H3K79ac Antibody

Recombinant H3K79ac Antibody [clone RM156] (R20206)

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  Catalog No Formulation Size Price (USD)  
Image R20206-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
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R20206-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 239
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Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K79ac antibody at 1 ug/ml showed a band of Histone H3 acetylated at Lysine 79 in treated HeLa cells.
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Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K79ac antibody at 1 ug/ml showed a band of Histone H3 acetylated at Lysine 79 in treated HeLa cells.
Sandwich ELISA of acetylated Histone H3 at Lys 79 using HeLa whole cell lysate, treated or untreated with sodium butyrate, with recombinant H3K79ac antibody (5 ug/ml) as the capture and biotinylated pan H3CT mAb (RM188, 1 ug/ml) as the detect.
ChIP performed on HeLa cells, with or without sodium butyrate treatment, using the recombinant H3K79ac antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.
ICC testing of HeLa cells treated with sodium butyrate using recombinant H3K79ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
The recombinant H3K79ac antibody specifically reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with acetylated Lysine 4/9/14/18/23/27/36/56/122 in Histone H3.
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM156
Purity Protein A purified from animal origin-free supernatant
UniProt P84243
Gene ID 8350
Applications Western blot : 0.5-2ug/ml
Immunocytochemistry : 0.5-2ug/ml
ChIP : 2-10ug/mg of lysate
ELISA : 0.2-1ug/ml
Limitations This recombinant H3K79ac antibody is available for research use only.
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Description

This recombinant H3K79ac antibody reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with other acetylated Lysines in Histone H3.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K79ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys79) was used as the immunogen for this recombinant H3K79ac antibody.

Storage

Store the recombinant H3K79ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Related Products

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   2.   View all H3K79ac antibodies

   3.   View all Histone H3 antibodies

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