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Home >> Antibodies >> Recombinant H2BK20ac Antibody

Recombinant H2BK20ac Antibody [clone RM235] (R20241)

  Catalog No Formulation Size Price (USD)  
Image R20241-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Image
R20241-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 239
Microvalidated Recrabbitmono
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Western blot test of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using recombinant H2BK20ac antibody at 0.5 ug/ml.
This recombinant H2BK20ac antibody specifically reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in Histone H2B.
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H2BK20ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM235
Purity Protein A purified from animal origin-free supernatant
UniProt P33778
Gene ID 3018
Applications Western blot : 0.5-2ug/ml
ELISA : 0.2-1ug/ml
Immunocytochemistry : 1-2ug/ml
Limitations This recombinant H2BK20ac antibody is available for research use only.
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Description

This recombinant H2BK20ac antibody reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H2BK20ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys20) was used as the immunogen for this recombinant H2BK20ac antibody.

Storage

Store the recombinant H2BK20ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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