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Home >> Antibodies >> Recombinant H2A.ZK4ac Antibody

Recombinant H2A.ZK4ac Antibody [clone RM221] (R20239)

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  Catalog No Formulation Size Price (USD)  
Image R20239-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 469
Image
R20239-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 239
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Western blot test of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using recombinant H2A.ZK4ac antibody at 0.5 ug/ml, showed a band of Histone H2A.Z acetylated at Lysine 4 in treated HeLa cells.
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Western blot test of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using recombinant H2A.ZK4ac antibody at 0.5 ug/ml, showed a band of Histone H2A.Z acetylated at Lysine 4 in treated HeLa cells.
This recombinant H2A.ZK4ac antibody specifically reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in Histone H2A.
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H2A.ZK4ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM221
Purity Protein A purified from animal origin-free supernatant
UniProt P0C0S5
Gene ID 3015
Applications Western blot : 0.5-2ug/ml
ELISA : 0.2-1ug/ml
Immunocytochemistry : 1-2ug/ml
Limitations This recombinant H2A.ZK4ac antibody is available for research use only.
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Description

This recombinant H2A.ZK4ac antibody reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H2A.ZK4ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H2A.Z (Lys4) was used as the immunogen for this recombinant H2A.ZK4ac antibody.

Storage

Store the recombinant H2A.ZK4ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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   2.   View all H2A.ZK4ac antibodies

   3.   View all Histone H2A.Z antibodies

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