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Home >> Antibodies >> Recombinant GFAP Antibody / Rabbit Monoclonal

Recombinant GFAP Antibody / Rabbit Monoclonal [clone GFA12-4R] (V3705)

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  Catalog No Formulation Size Price (USD)  
Image V3705-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3705-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3705SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3705IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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IHC testing of FFPE human cerebellum tissue with recombinant GFAP antibody (clone GFA12-4R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human cerebellum tissue with recombinant GFAP antibody (clone GFA12-4R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human. Other species not tested.
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name GFA12-4R
Purity Protein A affinity chromatography
UniProt P14136
Localization Cytoplasmic
Applications Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant GFAP antibody is available for research use only.
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Description

Glial fibrillary acidic protein is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [RefSeq]

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant GFAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Pig spinal cord GFAP was used as the immunogen for this recombinant GFAP antibody.

Storage

Store the recombinant GFAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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