Recombinant EpCAM Antibody / Extracellular domain [clone rEGP40/1372] (V3570)

IHC testing of FFPE human skin with recombinant EpCAM antibody (clone rEGP40/1372). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human rectal mass tissue with recombinant EpCAM antibody (clone rEGP40/1372). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone rEGP40/1372) as confirmation of integrity and purity.

Western blot testing of human MCF-7 cell lysate with recombinant EpCAM antibody (clone rEGP40/1372). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).

IHC staining of FFPE dog bladder with recombinant EpCAM antibody (clone rEGP40/1372). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE cat small intestine with recombinant EpCAM antibody (clone rEGP40/1372). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant EpCAM antibody (clone rEGP40/1372). These results demonstrate the foremost specificity of the rEGP40/1372 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.