- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Recombinant CK8 Antibody / Cytokeratin 8 [clone rB22.1] (V3560)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V3560-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 519 | |
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V3560-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 229 | |
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V3560SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 519 | |
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V3560IHC-7ML | Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* | 7 ml | 519 |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Recombinant Mouse Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone Name | rB22.1 |
| Purity | Protein G affinity chromatography |
| UniProt | P05787 |
| Localization | Cytoplasmic |
| Applications | Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT Western blot : 1-2ug/ml |
| Limitations | This recombinant CK8 antibody is available for research use only. |
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Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibody that recognizes only cytokeratin 8 and 18. Cytokeratin 8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium.
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CK8 antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
A cytoskeletal preparation from human HeLa cells was used as the immunogen for this recombinant CK8 antibody.
Store the recombinant CK8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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