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Home >> Antibodies >> Recombinant CK5 Antibody

Recombinant CK5 Antibody [clone RM226] (R20256)

  Catalog No Formulation Size Price (USD)  
Image R20256-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 0.1 ml 419
Microvalidated Recrabbitmono
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IHC staining of FFPE human tonsil tissue with recombinant CK5 antibody.
IHC staining of FFPE human tonsil tissue with recombinant CK5 antibody.
Western blot of A431 cell lysate with recombinant CK5 antibody. Predicted molecular weight 58~62 kDa.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Bovine, Mouse, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM226
Purity Protein A purified from animal origin-free supernatant
UniProt P13647
Applications Immunohistochemistry (FFPE) : 1:100 -1:400 (1)
Western blot : 1:1000
Limitations This recombinant CK5 antibody is available for research use only.
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Description

This recombinant CK5 antibody reacts to human Cytokeratin 5. It may also react to the bovine, mouse or rat protein, as predicted by immunogen homology.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant CK5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to the C-terminus of human Cytokeratin 5 was used as the immunogen for this recombinant CK5 antibody.

Storage

Store the recombinant CK5 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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