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Home >> Antibodies >> Recombinant CK20 Antibody / Cytokeratin 20 / KRT20

Recombinant CK20 Antibody / Cytokeratin 20 / KRT20 [clone RM283] (R20300)

  Catalog No Formulation Size Price (USD)  
Image R20300-0.1ML Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ul 419
Microvalidated Recrabbitmono
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IHC testing of FFPE human colon tissue with recombinant CK20 antibody at 1:250.
Western blot of human A431 lysate using recombinant CK20 antibody at 1:1000. Predicted molecular weight ~49 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM283
Purity Protein A purified from animal origin-free supernatant
UniProt P35900
Gene ID 54474
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:100-1:250 (1)
Western blot : 1:1000-1:2000
Limitations This recombinant CK20 antibody is available for research use only.
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Description

This recombinant CK20 antibody reacts to human CK20/Cytokeratin-20/Keratin 20.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant CK20 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

A peptide corresponding to the C-terminus of human Cytokeratin 20 was used as the immunogen for this recombinant CK20 antibody.

Storage

Store the recombinant CK20 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

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