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Home >> Antibodies >> Recombinant CK18 Antibody / Cytokeratin 18

Recombinant CK18 Antibody / Cytokeratin 18 [clone KRT18/2808R] (V7429)

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  Catalog No Formulation Size Price (USD)  
Image V7429-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7429-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7429SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7429IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC staining of FFPE human colon carinoma with recombinant CK18 antibody (clone KRT18/2808R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human colon carinoma with recombinant CK18 antibody (clone KRT18/2808R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human colon carinoma with recombinant CK18 antibody (clone KRT18/2808R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human thyroid carinoma with recombinant CK18 antibody (clone KRT18/2808R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Immunofluorescent staining of permeabilized human MCF7 cells with recombinant CK18 antibody antibody (clone KRT18/2808R, green) and Reddot nuclear stain (red).
Western blot testing of human HCT116 antibody with recombinant CK18 antibody (clone KRT18/2808R). Predicted molecular weight ~48 kDa.
Flow cytometry testing of permeabilized human HeLa cells with CK18 antibody (clone KRT18/2808R); Red=isotype control, Blue= anti-CK18 antibody.
SDS-PAGE analysis of purified, BSA-free recombinant CK18 antibody (clone KRT18/2808R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name KRT18/2808R
Purity Protein A affinity chromatography
UniProt P05783
Localization Cytoplasmic
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant CK18 antibody is available for research use only.
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Description

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, also called KRT18 and CK18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Application Notes

Optimal dilution of the recombinant CK18 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Full length recombinant human protein was used as the immunogen for the recombinant CK18 antibody.

Storage

Store the recombinant CK18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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