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Home >> Antibodies >> Recombinant CD86 Antibody

Recombinant CD86 Antibody [clone C86/2160R] (V3555)

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  Catalog No Formulation Size Price (USD)  
Image V3555-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3555-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3555SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3555IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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IHC testing of FFPE human colon with recombinant CD86 antibody (C86/2160R). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human colon with recombinant CD86 antibody (C86/2160R). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
IF/ICC staining of human Raji cells with recombinant CD86 antibody (green) and Reddot nuclear stain (red).
Western blot testing of human Ramos cell lysate with recombinant CD86 antibody. Expected molecular weight: 38-70 kDa depending on glycosylation level.
Flow cytometry testing of human Ramos cells with recombinant CD86 antibody (blue, clone C86/2160R) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free recombinant CD86 antibody (clone C86/2160R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name C86/2160R
Purity Protein A affinity chromatography
UniProt P42081
Localization Cytoplasmic, membrane
Applications ELISA : 2-4ug/ml (order BSA/azide-free format)
Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant CD86 antibody is available for research use only.
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Description

Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.

Application Notes

Optimal dilution of the recombinant CD86 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human full-length protein was used as the immunogen for the recombinant CD86 antibody.

Storage

Store the recombinant CD86 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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   2.   View all CD86 antibodies

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