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Home >> Antibodies >> Recombinant CD81 Antibody

Recombinant CD81 Antibody [clone rC81/3442] (V8525)

  Catalog No Formulation Size Price (USD)  
Image V8525-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8525-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8525SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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Flow cytometry staining of human MCF7 cells with recombinant CD81 antibody; Gray=unstained cells, Orange= recombinant CD81 antibody.
Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells using recombinant CD81 antibody (clone rC81/3442). Gray=unstained cells, Orange = recombinant CD81 antibody.
IHC staining of FFPE human tonsil with recombinant CD81 antibody (clone rC81/3442). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with recombinant CD81 antibody (clone rC81/3442). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rC81/3442
Purity Protein G affinity chromatography
UniProt P60033
Localization Cytoplasmic, membranous
Applications Functional Studies : order Ab format without BSA/sodium azide
Flow cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1ug/ml
Western blot : 1-2ug/ml for 2 hours at RT
Immunohistochemistry (FFPE) : 2-4ug/ml for 30 minutes at RT
Limitations This recombinant CD81 antibody is available for research use only.
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Description

Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Application Notes

Optimal dilution of the recombinant CD81 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human CD81 protein was used as the immunogen for the recombinant CD81 antibody.

Storage

Store the recombinant CD811 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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