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Home >> Antibodies >> Recombinant CD3e Antibody / CD3 epsilon

Recombinant CD3e Antibody / CD3 epsilon [clone C3e/4652R] (V9459)

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  Catalog No Formulation Size Price (USD)  
Image V9459-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9459-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9459SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human tonsil tissue with recombinant CD3e antibody (clone C3e/4652R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil tissue with recombinant CD3e antibody (clone C3e/4652R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymph node tissue with recombinant CD3e antibody (clone C3e/4652R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue using recombinant CD3e antibody (clone C3e/4652R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human Jurkat cell lysate using recombinant CD3e antibody (clone C3e/4652R). Predicted molecular weight ~23 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name C3e/4652R
Purity Protein A/G affinity
UniProt P07766
Localization Cell surface, Cytoplasm
Applications ELISA : 2-4ug/ml (order BSA-free format for coating)
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant CD3e antibody is available for research use only.
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Description

CD3 consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Application Notes

Optimal dilution of the recombinant CD3e antibody should be determined by the researcher.

Immunogen

A portion of amino acids aa 20-40 from the human protein was used as the immunogen for the recombinant CD3e antibody.

Storage

Aliquot the recombinant CD3e antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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