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Home >> Antibodies >> Recombinant CD3 epsilon Antibody

Recombinant CD3 epsilon Antibody [clone C3e/4653R] (V9434)

  Catalog No Formulation Size Price (USD)  
Image V9434-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9434-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9434SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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Western blot testing of human Jurkat cell lysate using recombinant CD3 epsilon antibody (clone C3e/4653R). Predicted molecular weight ~23 kDa.
IHC staining of FFPE human tonsil tissue with recombinant CD3e antibody (clone CD3e/4653R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue using recombinant CD3 epsilon antibody (clone C3e/4653R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name C3e/4653R
Purity Protein A/G affinity
UniProt P07766
Localization Cell Surface, Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western blot : 1-2ug/ml
Limitations This recombinant CD3 epsilon antibody is available for research use only.
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Description

Cluster of differentiation 3 (CD3) is a multimeric protein complex, known historically as the T3 complex, and is composed of four distinct polypeptide chains: epsilon, gamma, delta and zeta. The CD3 complex serves as a T cell co-receptor that associates noncovalently with the T cell receptor (TCR). CD3 antigen appears at all stages of T cell development which makes it an ideal T cell marker for both the detection of normal T cells and T cell neoplasms (lymphomas and leukemias).

Application Notes

Optimal dilution of the recombinant CD3 epsilon antibody should be determined by the researcher.

Immunogen

A portion of amino acids 100-200 was used as the immunogen for the recombinant CD3 epsilon antibody.

Storage

Aliquot the recombinant CD3 epsilon antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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