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Home >> Antibodies >> Recombinant CD21 Antibody

Recombinant CD21 Antibody [clone CR2/3124R] (V7754)

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  Catalog No Formulation Size Price (USD)  
Image V7754-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7754-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7754SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Immunofluorescent staining of PFA-fixed human MOLT4 cells with recombinant CD21 antibody (clone CR2/3124R, green) and Reddot nuclear stain (red).
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Immunofluorescent staining of PFA-fixed human MOLT4 cells with recombinant CD21 antibody (clone CR2/3124R, green) and Reddot nuclear stain (red).
Flow cytometry testing of PFA-fixed human MOLT4 cells with recombinant CD21 antibody (clone CR2/3124R); Red=isotype control, Blue= recombinant CD21 antibody.
IHC staining of FFPE human tonsil with recombinant CD21 antibody (clone CR2/3124R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD21 antibody (clone CR2/3124R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name CR2/3124R
Purity Protein A affinity chromatography
UniProt P20023
Localization Cell surface
Applications Flow cytometry : 1-2ug/10^6 cells in 0.1ml
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant CD21 antibody is available for research use only.
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Description

Recognizes a protein of 140kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.

Application Notes

Optimal dilution of the recombinant CD21 antibody should be determined by the researcher.

Immunogen

A partial protein corresponding to amino acids 142-240 was used as the immunogen for the recombinant CD21 antibody.

Storage

Store the recombinant CD21 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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