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Home >> Antibodies >> Recombinant CD146 Antibody

Recombinant CD146 Antibody [clone rMUC18/1130] (V3638)

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  Catalog No Formulation Size Price (USD)  
Image V3638-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3638-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3638SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3638IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recmousemono
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IHC testing of FFPE human tonsil with CD146 antibody (clone rMUC18/1130). Required boiling tissue sections in 10mM Tris with 1mM EDTA, pH8, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human tonsil with CD146 antibody (clone rMUC18/1130). Required boiling tissue sections in 10mM Tris with 1mM EDTA, pH8, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant CD146 antibody (clone rMUC18/1130) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rMUC18/1130
Purity Protein G affinity chromatography
UniProt P43121
Localization Cell surface, cytoplasm
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This recombinant CD146 antibody is available for research use only.
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Description

CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. It is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.

Application Notes

Optimal dilution of the recombinant CD146 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the recombinant CD146 antibody.

Storage

Store the recombinant CD146 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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