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Home >> Antibodies >> Recombinant Caldesmon Antibody / CALD1 / CAD (HMW)

Recombinant Caldesmon Antibody / CALD1 / CAD (HMW) [clone CALD1/1424R] (V7252)

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  Catalog No Formulation Size Price (USD)  
Image V7252-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7252-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7252SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7252IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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Western blot testing of human ovary tissue lysate using recombinant Caldesmon antibody (clone CALD1/1424R). Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle).
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Western blot testing of human ovary tissue lysate using recombinant Caldesmon antibody (clone CALD1/1424R). Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle).
IHC testing of FFPE human uterus with recombinant Caldesmon antibody (clone CALD1/1424R). Required HIER: boil tissue sections in 1mM EDTA, pH9, for 10-20 min.
IHC testing of FFPE rat uterus with recombinant Caldesmon antibody (clone CALD1/1424R). Required HIER: boil tissue sections in 1mM EDTA, pH9, for 10-20 min.
SDS-PAGE analysis of purified, BSA-free recombinant Caldesmon antibody (clone CALD1/1424R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name CALD1/1424R
Purity Protein A affinity chromatography
UniProt Q05682
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western blot : 1-2ug/ml
Limitations This recombinant Caldesmon antibody is available for research use only.
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Description

Caldesmon (CAD, CALD, CALD1) is an actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). [UniProt]

Application Notes

Titering of the recombinant Caldesmon antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A full length human protein was used as the immunogen for the recombinant Caldesmon antibody.

Storage

Store the recombinant Caldesmon antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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