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Home >> Antibodies >> Recombinant CALD1 Antibody/ Caldesmon / CAD (HMW)

Recombinant CALD1 Antibody/ Caldesmon / CAD (HMW) [clone rCALD1/7266] (V9402)

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  Catalog No Formulation Size Price (USD)  
Image V9402-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9402-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9402SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human smooth muscle with recombinant CALD1 antibody (clone rCALD1/7266). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human smooth muscle with recombinant CALD1 antibody (clone rCALD1/7266). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human smooth muscle tissue with recombinant CALD1 antibody (clone rCALD1/7266). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rCALD1/7266
Purity Protein A/G affinity
UniProt Q05682
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant CALD1 antibody is available for research use only.
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Description

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Application Notes

Optimal dilution of the recombinant CALD1 antibody should be determined by the researcher.

Immunogen

Recombinant human full-length CALD1 protein was used as the immunogen for the recombinant CALD1 antibody.

Storage

Aliquot the recombinant CALD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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