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Home >> Antibodies >> Recombinant BRAF V600E Antibody

Recombinant BRAF V600E Antibody [clone RM8] (R20306)

  Catalog No Formulation Size Price (USD)  
Image R20306-50UG 1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide 50 ug 429
Image
R20306-100UG 1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide 100 ug 679
Microvalidated Recrabbitmono
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Western blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein with recombinant BRAF V600E antibody.
IHC staining of formalin fixed and paraffin embedded melanoma tissue sections with recombinant BRAF V600E antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC staining of formalin fixed and paraffin embedded thyroid carcinoma tissue with recombinant BRAF V600E antibody. Allele specific PCR validated to be positive for BRAF V600E. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC staining of formalin fixed and paraffin embedded thyroid carcinoma tissue with recombinant BRAF V600E antibody. Allele specific PCR validated to be negative for BRAF V600E. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
ICC staining of formalin fixed and paraffin embedded human WiDr cells (400X, Positive) with recombinant BRAF V600E antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
ICC staining of formalin fixed and paraffin embedded human SW40 cells (400X, Negative) with recombinant BRAF V600E antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM8
Purity Protein A purified from animal origin-free supernatant
UniProt P15056
Localization Cytoplasmic, nuclear
Applications Immunohistochemistry (FFPE) : 0.5-5 ug/ml
Immunocytochemistry : 2-10 ug/ml
Western Blot : 0.5-2 ug/ml
Limitations This recombinant BRAF V600E antibody is available for research use only.
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Description

This antibody reacts to the BRAF V600E mutant. No cross reactivity seen with wild type BRAF.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant BRAF V600E antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A peptide corresponding to BRAF V600E mutant was used as the immunogen for the recombinant BRAF V600E antibody.

Storage

Store the recombinant BRAF V600E antibody at -20oC.

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