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Home >> Antibodies >> RBP4 Antibody / Retinol Binding Protein 4

RBP4 Antibody / Retinol Binding Protein 4 (R31773)

  Catalog No Formulation Size Price (USD)  
Image R31773 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of RBP4 antibody and Lane 1: rat liver; 2: mouse liver; 3: human HEPG2; 4: (h) COLO320 lysate. Predicted/observed molecular weight: ~23 kDa.
IHC staining of FFPE human liver cancer with RBP4 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with RBP4 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse kidney with RBP4 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse kidney with RBP4 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 5950
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
ELISA : 0.1-0.5ug/ml (human protein tested); request BSA free format for coating
Limitations This RBP4 antibody is available for research use only.
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Description

Retinol binding protein 4 (RBP4), also known as RDCCAS, belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of RBP4 posttranslationally and results in defective delivery and supply to the epidermal cells.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the RBP4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 19-201) was used as the immunogen for this RBP4 antibody.

Storage

After reconstitution, the RBP4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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