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- Tel: 858.663.9055
- Email: info@nsjbio.com
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EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.
Optimal dilution of the rat EpCAM antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The epitopes of mAbs Epcam/1158 and Epcam/1159 monoclonal antibodies are different.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Recombinant full-length rat protein was used as the immunogen for the rat EpCAM antibody.
Store the rat EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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