RAN Antibody / Ras-related nuclear protein (R32825)

Western blot testing of 1) rat brain, 2) rat testis, 3) rat thymus, 4) mouse brain, 5) mouse testis, 6) mouse thymus, 7) human A549, 8) human 22RV1 and 9) human HeLa lysate with RAN antibody at 0.5ug/ml. Predicted molecular weight ~25 kDa.

IHC testing of FFPE human breast cancer tissue with RAN antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE human intestinal cancer tissue with RAN antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE human lung cancer tissue with RAN antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE mouse small intestine tissue with RAN antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE rat small intestine tissue with RAN antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IF/ICC staining of FFPE human U-2 OS cells with RAN antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry testing of human A431 cells with RAN antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAN antibody.

Flow cytometry testing of human U937 cells with RAN antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAN antibody.