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Home >> Antibodies >> RACK1 Antibody

RACK1 Antibody (R31656)

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  Catalog No Formulation Size Price (USD)  
Image R31656 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of RACK1 antibody and Lane 1: mouse liver; 2: rat spleen; 3: (m) spleen; 4: human SMMC-7721; 5: (h) HEPG2; 6: (m) HEPA; 7: (r) RH35 lysate. Predicted molecular weight: ~35 kDa.
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Western blot testing of RACK1 antibody and Lane 1: mouse liver; 2: rat spleen; 3: (m) spleen; 4: human SMMC-7721; 5: (h) HEPG2; 6: (m) HEPA; 7: (r) RH35 lysate. Predicted molecular weight: ~35 kDa.
Western blot testing of RACK1 antibody and recombinant human protein (0.5ng)
Immunofluorescent staining of FFPE human A431 cells with RACK1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A431 cells with RACK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RACK1 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 10399
Applications Western blot : 0.5-1ug/ml
Immunofluorescence (FFPE) : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This RACK1 antibody is available for research use only.
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  • Applications : WB, ELISA (peptide)
    Reactivity : Human, Mouse, Rat
    Pred. Reactivity : Cow, Dog

Description

Guanine nucleotide-binding protein subunit beta-2-like 1, also known as RACK1, is a 35kDa protein that in humans is encoded by the GNB2L1 gene. It is mapped to 5q35.3. It is a major component of translating ribosomes, which harbor significant amounts of PKC. This gene can provide a physical and functional link between PKC signaling and ribosome activation. GNB2L1/RACK1 is also a mediator of agonist-induced Ca(2+) release. Additionally, it is an essential component of an oxygen-independent mechanism for regulating HIF1A stability. Overexpression of RACK1 and PKC-alpha suppressed CLOCK-BMAL1 transcriptional activity, and it stimulated phosphorylation of BMAL1 by PKC-alpha in vitro.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the RACK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 2-317) was used as the immunogen for this RACK1 antibody.

Storage

After reconstitution, the RACK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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