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Home >> Antibodies >> RAB9 Antibody

RAB9 Antibody (R31405)

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  Catalog No Formulation Size Price (USD)  
Image R31405 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of RAB9 antibody and Lane 1: rat brain; 2: mouse brain; 3: human HeLa; 4: (r) PC12; 5: (m) NIH3T3; 6: (h) A431; 7: (h) 293T lysate. Expected/observed molecular weight: ~23 kDa.
Western blot testing of RAB9 antibody and Lane 1: rat brain; 2: mouse brain; 3: human HeLa; 4: (r) PC12; 5: (m) NIH3T3; 6: (h) A431; 7: (h) 293T lysate. Expected/observed molecular weight: ~23 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P51151
Applications Western blot : 0.5-1ug/ml
Limitations This RAB9 antibody is available for research use only.
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Description

Ras-related protein Rab9A, also called Rab9, is a protein that in humans is encoded by the RAB9A gene. Rab9 has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors. It has been found that downregulation of RAB9A gene expression in HeLa cells induced severe cell vacuolation. RAB9A GTPase is directly bound by TIP47 in its active, GTP-bound conformation. Moreover, the protein increases the affinity of TIP47 for its cargo. Additionally, this gene may involved in the transport of proteins between the endosomes and the trans Golgi network. RAB9A has been shown to interact with RABEPK and TIP47.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the RAB9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of human RAB9A (RSDHLIQTDTVNLHRK) was used as the immunogen for this RAB9 antibody (100% rat homology).

Storage

After reconstitution, the RAB9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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