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Home >> Antibodies >> RAB5A/B/C Antibody

RAB5A/B/C Antibody (RQ7003)

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  Catalog No Formulation Size Price (USD)  
Image RQ7003 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human MCF7 cells with RAB5A/B/C antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human MCF7 cells with RAB5A/B/C antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lymphoma tissue with RAB5A/B/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with RAB5A/B/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with RAB5A/B/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human adenocarcinoma of the right colon tissue with RAB5A/B/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cell carcinoma tissue with RAB5A/B/C antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human U-87 MG, 2) human MCF7, 3) human U-251, 4) human HL60, 5) rat kidney, 6) rat testis and 7) mouse kidney tissue lysate with RAB5A/B/C antibody. Predicted molecular weight: 23-25 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P20339 , P61020 , P51148
Localization Cytoplasmic
Applications Western blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This RAB5A/B/C antibody is available for research use only.
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Description

Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment.

Application Notes

Optimal dilution of the RAB5A/B/C antibody should be determined by the researcher.

Immunogen

Recombinant human RAB5A/B/C protein (amino acids M1-N215) was used as the immunogen for the RAB5A/B/C antibody.

Storage

After reconstitution, the RAB5A/B/C antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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