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Home >> Antibodies >> PTEN Antibody

PTEN Antibody [clone PTEN/7344] (V4110)

  Catalog No Formulation Size Price (USD)  
Image V4110-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4110-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4110SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human prostate carcinoma tissue with PTEN antibody (clone PTEN/7344) at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free PTEN antibody (clone PTEN/7344) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG, kappa
Clone Name PTEN/7344
Purity Protein A/G affinity
UniProt P60484
Localization Cytoplasm, Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This PTEN antibody is available for research use only.
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Description

PTEN (phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase) contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. It was identified as a tumor suppressor that is mutated in a large number of cancers, including sporadic brain, breast, kidney, and prostate cancers.

Application Notes

Optimal dilution of the PTEN antibody should be determined by the researcher.

Immunogen

Recombinant full-length human PTEN protein was used as the immunogen for the PTEN antibody.

Storage

Aliquot the PTEN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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