PSMB4 Antibody / Proteasome subunit beta type 4 (RQ7242)

IHC staining of FFPE human ovarian cancer tissue with PSMB4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast cancer tissue with PSMB4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human esophageal squamous carcinoma tissue with PSMB4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colorectal adenocarcinoma tissue with PSMB4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with PSMB4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat stomach tissue with PSMB4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human breast cancer tissue with PSMB4 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Immunofluorescent staining of FFPE human intestinal cancer tissue with PSMB4 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Western blot testing of 1) human HeLa, 2) human HepG2, 3) human A431, 4) human RT4, 5) human U-251, 6) human placenta, 7) human MCF7, 8) rat skeletal muscle, 9) mouse liver and 10) mouse skeletal muscle tissue lysate with PSMB4 antibody. Predicted molecular weight ~29 kDa, commonly observed at 26-29 kDa.

Flow cytometry testing of human HEL cells with PSMB4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSMB4 antibody.