PSF Antibody / PTB-associated-splicing factor / SFPQ (RQ7421)

IHC staining of FFPE human ovarian cancer tissue with PSF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with PSF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human squamous cell carcinoma of the penis with PSF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse brain with PSF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat brain with PSF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human ovarian cancer tissue with PSF antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Immunofluorescent staining of FFPE mouse brain cancer tissue with PSF antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Immunofluorescent staining of FFPE human MCF7 cells with PSF antibody (green) and Alpha Tubulin antibody (red). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human HeLa, 2) human 293T, 3) human HepG2, 4) human PC-3, 5) rat brain, 6) mouse brain, 7) mouse C2C12 and 8) mouse NIH 3T3 cell lysate with PSF antibody. Predicted molecular weight ~76 kDa, commonly observed at 90-100 kDa with possible 47 kDa and 68 kDa cleavage products.

Flow cytometry testing of human SiHa cells with PSF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSF antibody.

Flow cytometry testing of mouse ANA-1 cells with PSF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSF antibody.

Flow cytometry testing of rat RH35 cells with PSF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PSF antibody.