• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> PROM1 Antibody / CD133

PROM1 Antibody / CD133 [clone 8B6] (RQ6236)

Pdf Image
  Catalog No Formulation Size Price (USD)  
Image RQ6236 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
Bulk quote request
Flow cytometry testing of human Caco-2 cells with PROM1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PROM1 antibody.
›
Flow cytometry testing of human Caco-2 cells with PROM1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PROM1 antibody.
Western blot testing of 1) human Caco-2, 2) human SW620 and 3) mouse kidney lysate with PROM1 antibody. Expected molecular weight: 97 kDa-130 kDa depending on glycosylation level.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Clone Name 8B6
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O43490
Applications Western blot : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This PROM1 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

Prominin-1, also known as CD133, is a glycoprotein that in humans is encoded by the PROM1 gene. It is mapped to 4p15.32. Prominin-1 is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. It has been proposed to act as an organizer of cell membrane topology. Prominin-1 was expressed not only on metastatic colon cancer cells, but also on differentiated colonic epithelium in both adult mice and humans.

Application Notes

Optimal dilution of the PROM1 antibody should be determined by the researcher.

Immunogen

A human recombinant partial protein (amino acids P531-H865) was used as the immunogen for the PROM1 antibody.

Storage

After reconstitution, the PROM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all PROM1 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.