PP2A Antibody (catalytic subunit) / PPP2CA (R31760)

Immunofluorescent staining of FFPE human U-2 OS cells with PP2A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

IHC staining of FFPE human thyroid cancer tissue with PP2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human esophageal squamous carcinoma tissue with PP2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colorectal adenocarcinoma tissue with PP2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse brain tissue with PP2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat brain tissue with PP2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human Jurkat, 2) human MCF7, 3) human 293T, 4) human HeLa, 5) human HepG2, 6) rat brain, 7) rat PC-12, 8) mouse lung, 9) mouse liver, 10) mouse brain and 11) mouse NIH 3T3 cell lysate with PP2A antibody. Predicted molecular weight ~35 kDa.

Flow cytometry testing of fixed and permeabilized human K562 cells with PP2A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PP2A antibody.