PNPLA6 Antibody / NTE (RQ8014)

PNPLA6 Antibody for IF. Immunofluorescence analysis in human U-2 OS cells. FFPE human U-2 OS cells stained with PNPLA6 antibody (red) show predominantly cytoplasmic staining, consistent with its role as a membrane-associated phospholipase. Nuclei are counterstained with DAPI (blue). Antigen retrieval was performed using pH6 citrate buffer with heat-induced epitope retrieval prior to staining.

PNPLA6 Antibody for WB. Western blot analysis in human HeLa, 293T, and SH-SY5Y cell lysates. Lane 1: HeLa lysate, Lane 2: 293T lysate, Lane 3: SH-SY5Y lysate. A band is detected at approximately 140-150 kDa, consistent with the predicted molecular weight of Patatin-like phospholipase domain-containing protein 6 / PNPLA6. The observed band pattern likely reflects multiple isoforms of PNPLA6, which are known to arise from alternative splicing and contribute to its diverse roles in phospholipid metabolism and neuronal maintenance.

PNPLA6 Antibody for FACS. Flow cytometry analysis in human SiHa cells. Fixed and permeabilized human SiHa cells were stained with PNPLA6 antibody at 1 ug per million cells following blocking with goat sera. Red histogram represents unstained cells, green indicates isotype control, and blue shows PNPLA6 antibody staining with a clear rightward shift, demonstrating specific detection of Patatin-like phospholipase domain-containing protein 6 / PNPLA6. This result supports intracellular expression of PNPLA6 consistent with its role in phospholipid metabolism and neuronal-associated signaling pathways.

PNPLA6 Antibody for IHC. Immunohistochemistry analysis in human brain tissue. FFPE human brain tissue stained with PNPLA6 antibody shows cytoplasmic staining in neuronal cell bodies and processes, consistent with its role in phospholipid metabolism and neuronal maintenance. Detection was performed using an HRP-conjugated secondary antibody with DAB chromogen (brown), and nuclei are counterstained with hematoxylin (blue). Antigen retrieval was carried out using pH8 EDTA buffer with heat-induced epitope retrieval prior to staining.