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Home >> Antibodies >> PKC eta Antibody

PKC eta Antibody (R32029)

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  Catalog No Formulation Size Price (USD)  
Image R32029 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
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Western blot testing of human 1) A431, 2) A549, 3) HeLa and 4) SKOV lysate with PKC eta antibody. Predicted/observed molecular weight ~78 kDa.
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Western blot testing of human 1) A431, 2) A549, 3) HeLa and 4) SKOV lysate with PKC eta antibody. Predicted/observed molecular weight ~78 kDa.
Flow cytometry testing of human A431 cells with PKC eta antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PKC eta antibody.
Flow cytometry testing of human MCF7 cells with PKC eta antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PKC eta antibody.
Flow cytometry testing of human K562 cells with PKC eta antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PKC eta antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P24723
Localization Cytoplasmic
Applications Western blot : 0.1-0.5ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Limitations This PKC eta antibody is available for research use only.
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Description

PRKCH is also known as PKC eta. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. Also, PKC family members serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. And it is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.

Application Notes

Optimal dilution of the PKC eta antibody should be determined by the researcher.

Immunogen

Amino acids 30-389 of human PKC eta were used as the immunogen for the PKC eta antibody.

Storage

After reconstitution, the PKC eta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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