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Home >> Antibodies >> PIP Antibody / Prolactin Inducible Protein / GCDFP-15

PIP Antibody / Prolactin Inducible Protein / GCDFP-15 [clone PIP/1571] (V3281)

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  Catalog No Formulation Size Price (USD)  
Image V3281-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3281-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3281SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE human breast carcinoma with PIP antibody (clone PIP/1571). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20.
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IHC testing of FFPE human breast carcinoma with PIP antibody (clone PIP/1571). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20.
Western blot testing of human 1) pancreas and 2) HepG2 lysate with PIP antibody (clone PIP/1571). Expected molecular weight ~15 kDa.
SDS-PAGE Analysis of Purified, BSA-Free PIP Antibody (clone PIP/1571). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name PIP/1571
Purity Protein G affinity chromatography
UniProt P12273
Localization Cytoplasmic (Golgi bodies), secreted
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 0.5-1ug/ml
Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This PIP antibody is available for research use only.
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Description

It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15), also called Prolactin Inducible Protein, or PIP. It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. PIP is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.

Application Notes

Titering of the PIP antibody may be required for optimal performance.

Immunogen

A human partial recombinant protein corresponding to amino acids 41-146 was used as the immunogen for the PIP antibody.

Storage

Store the PIP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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