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Home >> Antibodies >> Phosphotyrosine Antibody

Phosphotyrosine Antibody [clone 7B4-D9-H10] (F54032)

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  Catalog No Formulation Size Price (USD)  
Image F54032-0.1ML In PBS with 50% glycerol and 0.03% ProClin 300 0.1 ml 399
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Western blot analysis of extracts from A549 cells, untreated and treated with Epidermal Growth Factor (10ng/ml, 15min), using Phosphotyrosine antibody. The phospho-specificity of the antibody was further characterized by treating the membrane with alkaline phosphatase after Western transfer.
Western blot analysis of extracts from A549 cells, untreated and treated with Epidermal Growth Factor (10ng/ml, 15min), using Phosphotyrosine antibody. The phospho-specificity of the antibody was further characterized by treating the membrane with alkaline phosphatase after Western transfer.
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Clone Name 7B4-D9-H10
Purity Protein G affinity
Applications Western blot : 1:1000
Limitations This Phosphotyrosine antibody is available for research use only.
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Description

Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Phosphotyrosine antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A phospho-tyrosine containing peptide was used as the immunogen for this Phosphotyrosine antibody.

Storage

Store the Phosphotyrosine antibody at -20oC.

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