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Home >> Antibodies >> Phospho-SMAD3 Antibody (pS208)

Phospho-SMAD3 Antibody (pS208) (F48449)

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  Catalog No Formulation Size Price (USD)  
Image F48449-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48449-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Confocal immunofluorescent analysis of phospho-SMAD3 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
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Confocal immunofluorescent analysis of phospho-SMAD3 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
Dot blot analysis of phospho-SMAD3 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Chicken, Mouse, Pig, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P84022
Applications Immunofluorescence : 1:10-1:100
Dot blot : 1:500
Limitations This phospho-SMAD3 antibody is available for research use only.
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Description

SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80% of all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes. SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes. Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma.

Application Notes

Titration of the phospho-SMAD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This phospho-SMAD3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS208 of human SMAD3.

Storage

Aliquot the phospho-SMAD3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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