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Home >> Antibodies >> Phospho-KDR Antibody / VEGFR2 (pY996)

Phospho-KDR Antibody / VEGFR2 (pY996) (F48399)

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  Catalog No Formulation Size Price (USD)  
Image F48399-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48399-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Phospho-KDR antibody used in western blot to detect phosphorylated KDR/FLK1 in HMVEC lysate. Endothelial cells were stimulated with 50ug/ml VEGF for 5min and Ab used at 1:500. Predicted molecular weight: ~152 (immature), 180-200 kDa (intermediate) and 220-230 kDa (mature).
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Phospho-KDR antibody used in western blot to detect phosphorylated KDR/FLK1 in HMVEC lysate. Endothelial cells were stimulated with 50ug/ml VEGF for 5min and Ab used at 1:500. Predicted molecular weight: ~152 (immature), 180-200 kDa (intermediate) and 220-230 kDa (mature).
IHC analysis of FFPE human hepatocarcinoma tissue stained with the phospho-KDR antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P35968
Localization Cytoplasmic and cell surface
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Limitations This phospho-KDR antibody is available for research use only.
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Description

KDR (FLK1) is a receptor for VEGF or VEGFC. This protein has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability.

Application Notes

Titration of the phospho-KDR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This phospho-KDR antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY996 of human KDR/FLK1.

Storage

Aliquot the phospho-KDR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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