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Home >> Antibodies >> Phospho-Histone H3 Antibody (pS28)

Phospho-Histone H3 Antibody (pS28) (F40127)

  Catalog No Formulation Size Price (USD)  
F40127-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 299

F40127-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 125
Western blot analysis of Histone H3 antibody and HepG2 lysate.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P68431
Applications Western blot : 1:1000
Limitations This phospho-Histone H3 antibody is available for research use only.

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Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Application Notes

Titration of the phospho-Histone H3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This phospho-H3 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS28 of human H3.

Storage

Aliquot the phospho-Histone H3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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