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Home >> Antibodies >> Phospho-eNos Antibody / NOS3 (pS1177)

Phospho-eNos Antibody / NOS3 (pS1177) (F48608)

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  Catalog No Formulation Size Price (USD)  
Image F48608-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48608-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Dot blot analysis of phospho-eNos antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
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Dot blot analysis of phospho-eNos antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
Confocal immunofluorescent analysis of phospho-eNos antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Bovine, Mouse, Pig, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P29474
Applications Dot blot : 1:500
Immunofluorescence : 1:10-1:50
Limitations This phospho-eNos antibody is available for research use only.
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Description

eNos produces nitric oxide, a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases.

Application Notes

Titration of the phospho-eNos antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This phospho-eNos antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS1177 of human eNos.

Storage

Aliquot the phospho-eNos antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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