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Home >> Antibodies >> Phospho c-Myc Antibody (pT58)

Phospho c-Myc Antibody (pT58) (F48482)

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  Catalog No Formulation Size Price (USD)  
Image F48482-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48482-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of phospho c-Myc antibody and human TPA activated HeLa cells/lysate (0: without TPA; 1: 60ug/ml TPA-15min; 2: 60ug/ml-30min; 3: 60ug/ml-45min; 4: 125ug/ml-15min; 5: 125ug/ml-30min; 6: 125ug/ml-45min; 7: 250ug/ml-15min; 8: 250ug/ml-30min; 9: 250ug/ml-45min)
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Western blot analysis of phospho c-Myc antibody and human TPA activated HeLa cells/lysate (0: without TPA; 1: 60ug/ml TPA-15min; 2: 60ug/ml-30min; 3: 60ug/ml-45min; 4: 125ug/ml-15min; 5: 125ug/ml-30min; 6: 125ug/ml-45min; 7: 250ug/ml-15min; 8: 250ug/ml-30min; 9: 250ug/ml-45min)
Dot blot analysis of phospho c-Myc antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Bovine, Chicken, Mouse, Pig, Rat, Xenopus, Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P01106
Applications Dot blot : 1:500
Western blot : 1:1000
Limitations This phospho c-Myc antibody is available for research use only.
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Description

c-Myc participates in the regulation of gene transcription. It binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. This protein appears to activate the transcription of growth-related genes. Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia.

Application Notes

Titration of the phospho c-Myc antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This phospho c-Myc antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT58 of human MYC.

Storage

Aliquot the phospho c-Myc antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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