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Home >> Antibodies >> Phospho-c-Jun Antibody (pT91/93)

Phospho-c-Jun Antibody (pT91/93) [clone C-J 4C4/1] (V7427)

  Catalog No Formulation Size Price (USD)  
Image V7427-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7427-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7427SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7427IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC testing of FFPE human cervical tumor with phospho-c-Jun antibody (clone C-J 4C4/1). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human cervical tumor with phospho-c-Jun antibody (clone C-J 4C4/1). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free phospho-c-Jun antibody (clone C-J 4C4/1) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name C-J 4C4/1
Purity Protein G affinity chromatography
UniProt P05412
Localization Nuclear
Applications Flow cytometry : 0.5-1ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This phospho-c-Jun antibody is available for research use only.
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Description

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [RefSeq]

Application Notes

Optimal dilution of the phospho-c-Jun antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

The amino acids surrounding phosphorylated T91 and T93 were used as the immunogen for the phospho-c-Jun antibody.

Storage

Store the phospho-c-Jun antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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