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Home >> Antibodies >> Phospho-BRAF Antibody (pT400)

Phospho-BRAF Antibody (pT400) (F48555)

  Catalog No Formulation Size Price (USD)  
Image F48555-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48555-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Dot blot analysis of phospho-BRAF antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P15056
Applications Dot blot : 1:500
Limitations This phospho-BRAF antibody is available for research use only.
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Description

BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome), and a wide range of cancers such as lung cancer, non-Hodgkins lymphoma, and colorectal cancer.

Application Notes

Titration of the phospho-BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This phospho-BRAF antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT400 of human BRAF.

Storage

Aliquot the phospho-BRAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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