• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> Phos-ERBB2 Antibody (pY1127)

Phos-ERBB2 Antibody (pY1127) (F48692)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image F48692-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48692-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
Bulk quote request
Western blot analysis of extracts from A431 cells, untreated or treated with EGF (100ng/ml), using phos-ERBB2 antibody (left) or nonphos Ab (right)
›
Western blot analysis of extracts from A431 cells, untreated or treated with EGF (100ng/ml), using phos-ERBB2 antibody (left) or nonphos Ab (right)
Fluorescent confocal image of MCF7 cells stained with phos-ERBB2 antibody at 1;100. Note the highly specific localization to the plasma membrane and cytoplasm.
Dot blot analysis of phos-ERBB2 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P04626
Applications Western blot : 1:1000-5000
Immunofluorescence : 1:100
Dot blot : 1:500
Limitations This phos-ERBB2 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Application Notes

Titration of the phos-ERBB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This phos-ERBB2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1127 of human HER2/ERBB2.

Storage

Aliquot the phos-ERBB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Related Products

   1.   View all Phos-ERBB2 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.