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Home >> Antibodies >> PHB Antibody

PHB Antibody (F48124)

  Catalog No Formulation Size Price (USD)  
Image F48124-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48124-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC analysis of FFPE human hepatocarcinoma tissue stained with PHB antibody
Western blot testing of PHB antibody and HeLa cell lysate. Predicted molecular weight ~28 kDa.
Confocal immunofluorescent analysis of PHB antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
PHB antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Bovine, Chicken
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P35232
Applications IHC (Paraffin) : 1:10-1:50
Western blot : 1:1000
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This PHB antibody is available for research use only.
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Description

Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region.

Application Notes

Titration of the PHB antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 89-117 from the human protein was used as the immunogen for this PHB antibody.

Storage

Aliquot the PHB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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