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Home >> Antibodies >> PGK1 Antibody

PGK1 Antibody (F40146)

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  Catalog No Formulation Size Price (USD)  
Image F40146-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F40146-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of lysate from A431, HeLa, U-937, BA/F3 cell lines using PGK1 diluted at 1:1000 for each lane. Expected/observed molecular weight ~44kDa.
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Western blot analysis of lysate from A431, HeLa, U-937, BA/F3 cell lines using PGK1 diluted at 1:1000 for each lane. Expected/observed molecular weight ~44kDa.
IHC analysis of FFPE human hepatocarcinoma tissue stained with PGK1 antibody
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Primate
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P00558
Localization Nuclear, cytoplasmic
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Limitations This PGK1 antibody is available for research use only.
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Description

Also known as ATP:3-phosphoglycerate 1-phosphotransferase, this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. Phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and is proposed to participate in the angiogenic process as a disulfide reductase. Mutations in PGK1 may be associated with hemolytic anemia.

Application Notes

Titration of the PGK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 305-334 from the human protein was used as the immunogen for this PGK1 antibody.

Storage

Aliquot the PGK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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