PDIA6 Antibody | ERp5| RQ7022 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	RQ7022	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	429
Formulation

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IHC staining of FFPE human breast infiltrating ductal carcinoma tissue with PDIA6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human ovarian cancer tissue with PDIA6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human bladder epithelial carcinoma tissue with PDIA6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human placental tissue with PDIA6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with PDIA6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with PDIA6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human endometrial cancer tissue with PDIA6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colorectal adenocarcinoma tissue with PDIA6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human HepG2 cells with PDIA6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human K562, 2) human HepG2, 3) human HT1080 and 4) monkey COS-7 cell lysate with PDIA6 antibody. Predicted molecular weight ~48 kDa.

Flow cytometry testing of human SiHa cells with PDIA6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PDIA6 antibody.

Availability	1-3 business days
Species Reactivity	Human, Monkey
Format	Antigen affinity purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG2b
Clone Name	3H5E7
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	Q15084
Localization	Cytoplasmic, cell membrane
Applications	Western blot : 0.5-1 ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Flow cytometry : 1-3ug/million cells
Limitations	This PDIA6 antibody is available for research use only.
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Description

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. This protein inhibits the aggregation of misfolded proteins and exhibits both isomerase and chaperone activity. Alternative splicing results in multiple transcript variants encoding different isoforms.

Application Notes

Optimal dilution of the PDIA6 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids L20-L440) was used as the immunogen for the PDIA6 antibody.

Storage

After reconstitution, the PDIA6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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PDIA6 Antibody / ERp5 [clone 3H5E7] (RQ7022)

Description

Application Notes

Immunogen

Storage

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Area of Research

Product Type

Protocols

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