PC4 Antibody / Positive cofactor 4 / SUB1 [clone 8D9D1] (RQ7288)

IHC staining of FFPE human hepatocellular carcinoma tissue with PC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung adenocarcinoma tissue with PC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with PC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colorectal adenocarcinoma tissue with PC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast cancer tissue with PC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human testicular germ cell tumor tissue with PC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse liver tissue with PC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat liver tissue with PC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human HeLa, 2) human A549, 3) human MCF7, 4) human T-47D, 5) rat liver, 6) rat spleen and 7) mouse spleen tissue lysate with PC4 antibody. Expected molecular weight: 15-19 kDa (unmodified) and ~26 kDa (phosphorylated).

Immunofluorescent staining of FFPE human intestinal cancer tissue with PC4 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Flow cytometry testing of human HepG2 cells with PC4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PC4 antibody.