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Home >> Antibodies >> PAX-6 Antibody

PAX-6 Antibody (F49968)

  Catalog No Formulation Size Price (USD)  
Image F49968-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F49968-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of PAX-6 in Y79 lysate. Predicted molecular weight ~48kDa.
Western blot analysis of PAX-6 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the human gene.
PAX-6 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue
Confocal immunofluorescent analysis of PAX-6 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P26367
Localization Nuclear, cytoplasmic
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Limitations This PAX-6 antibody is available for research use only.
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Description

Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). [UniProt]

Application Notes

Titration of the PAX-6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 352-380 from the human protein was used as the immunogen for this PAX-6 antibody.

Storage

Aliquot the PAX-6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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